linking affinity purification protocol (cCLAP) based on existing.
Use the tubes that are recommended by the company (screwcaps). Therefore, you need to take 1.2 µg of DNA from your original sample, and complete it to 15 µl with either pure water, either 10 mM Tris-HCl (pH 8), either 10 mM Tris-HCl (pH 8) with a maximum of 0.01 mM EDTA, depending on your needs. The plasmid concentration should be 80 ng/µl, and the total volume shall be 15 µl. Download: PPT PowerPoint slide PNG larger image TIFF original image Table 1. DNA/RNA SYNTHESIS DNA/RNA ANALYSYS AND SEQUENCING CONTRACT RESEARCH/OUTSOURCING. Primers were ordered HPL-purified from Microsynth AG (Balgach, Switzerland). PARTICIPATE IN THE EXHIBITOR QUIZ Which PCR platforms does Microsynth. Prepare the DNA of interest according to Microsynth guidelines. The sequences of all primers are listed in Table 1.Log in to the Microsynth website, place your order and print out the primer barcode. If you get the expected product, they are correct.
Only 45 EUR per sample for a minimum of 8 samples and even lower prices for higher numbers.
#Microsynth sequencing sample preparation serial#
Test your primers by running a virtual PCR with Serial Cloner or any other software on your sequence. Take the opportunity and try our 16S/ITS sequencing service at a great rate.You can also order the primers from some other company and send them along with the DNA sample. Most cost-efficient Sanger sequencing service in tubes from Microsynth. If there is a standard sequence in that area, pick a pre-made primer from the standard list. Design the sequencing primers that should start approximately 50 bp upstream and downstream of your sequence of interest. Biosystems), 4M of the primers ordered from Microsynth (Balgach) and 22.5 ng of the previously.It also has a library of standard primers, in case there is some kind of common sequence in the vicinity of the sequence of interest that could be used as the starting point for Sanger sequencing. Microsynth is a Swiss sequencing company that has a pick-up service at EPFL, which means the sample doesn't need to be sent anywhere.
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